This is a method to pick 48 or more individual colonies from a plate of antibiotic-selected cells in 15 minutes without the use of those tedious cloning rings.

It is based on bacterial plate agarose overlay methods. We have been using this method for over 20 years in the lab with excellent success, making over 300 cell lines.

Watch Dr. Lindberg’s tutorial video (if embedded video does not load, watch it on YouTube here)

Click to download the written protocol in .doc format